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After centrifuging the plates for 5 minutes at 1,500 rpm, duplicate wells were harvested and lysed with 350 l of RLT buffer

Vitamins like vitamin D, B6, B12, and folate are among the most frequently used

However, it remains unapproved by the FDA and banned for human use in many countries due to the lack of human trials and concerns over safety, contamination, and legal misuse