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Parallel studies using primary isolated mouse hepatocytes showed that acute inhibition of VCP activity with either the drug DBeQ, or siRNA mediated knockdown, resulted in an elevation of HSD1713 levels and a 5-fold increase in LD content

FIGURE 2 The Light-Sensitive Mutant Combining the Deletion of slr0236 and the Depletion of sll1545 Exposed to High-Light Undergoes a Transient Sequential Accumulation of ROS and Then of Reduced (GSH) and Oxidized (GSSG) Glutathione To analyze the influence of high-light on the mutants slr0236 (absence of slr0236 ), sll1545 (depletion of sll1545 ), slr0236-sll1545 (combined absence of slr0236 and depletion of sll1545 ) and the WT strain we measured the ROS content (Figures 3A,B), using the standard fluorescent probe DCHF-DA ( total = GSH + GSSG) (Figures 3G,H) using the relevant standard assay ( slr0236-sll1545 mutant facing high light (7500 lux) rapidly (in 1 h) accumulated ROS (Figure 3B)

Their main role is to deal with the stress-induced injury of cells (Antonioli et al., 2017)

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