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The molecular changes were characterized in depth by scRNA-seq analysis of crypto and normal testicular biopsies (n = 3 each) to assess the similarities and differences in the two sets of groups

However, a growing body of evidence reveals a pronounced immunoinflammatory phenotype in OA, wherein macrophages, T/B lymphocytes, and inflammatory factors such as IL-1, TNF-, and IL-6 play a critical role in the local microenvironment ( 4.1 The role of immune cells in the local microenvironment of OA Macrophages are among the most prominent immune cells within the OA synovium

The PBS was then aspirated, and cells were resuspended in BMDM media with 30% FBS and 10% DMSO at 10 7 cells/ml