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Analysis of biological thiols: derivatization with monobromobimane and separation by reverse phase high-performance liquid chromatography

GeneTailor Site-Directed Mutagenesis System (Invitrogen) was used to perform site-directed mutagenesis of the miR-221 binding site in the IGF-1 3'UTR (designated as mut)

Cell Death Differ 27(2):662675 Wang S, Liu W, Wang J, Bai X (2020b) Curculigoside inhibits ferroptosis in ulcerative colitis through the induction of GPX4

The results indicated that certain regions within the PPI network exhibited significantly enhanced interactions, and mitochondrial-related proteins with significant causal effects on DKA identified in current analyses were primarily concentrated in these regions (Figure 5B)