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Mitochondrial dysfunction and mitophagy in Parkinsons: from familial to sporadic disease

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Common Symptoms Tolerance: Requiring progressively higher Adderall doses to achieve the same cognitive enhancement or mood effect, reflecting DRD2 downregulation in the mesolimbic pathway

Subsequently, slides were washed 3X with PBS (10 min, RT) and incubated with the secondary horseradish peroxidase conjugated antibody (mouse anti-rabbit IgG, 0.4 mg/mL diluted 1:500, sc-2357 or goat anti-mouse IgG, 0.4 mg/mL diluted 1:500, sc-2031) for 2 h at RT, followed by three PBS washes and treated with the 3,3-diaminobenzidine substrate (ab64238, Abcam) for 10 min