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Cells were washed three times with ice-cold PBS and lysed in lysis buffer (40 mM Tris-HCl (pH 8.0), 1% Triton X-100, 1% NP-40, 10% glycerol, 0.15 M NaCl, 2 mM EDTA, 1 mM phenylmethanesulfonyl fluoride, 1 Complete protease inhibitor cocktail)

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doi:10.2174/1874312901206010273

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12 ] In humans, melatonin production often declines with age, which may contribute to sleep disturbances and disruptions in normal biological timing

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