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RNA Isolation Cells were washed with PBS without Ca 2+ and Mg 2+ (Sigma) and each sample (1 10 6 cells) was lyzed with 1 ml QIAzol (QIAGEN)

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Clinical Takeaway: Personalised Nutritional and Lifestyle Support Understanding a patients MTHFR status allows for personalised interventions that address root biochemical dysfunctions rather than just symptoms