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Centrifuge the homogenized tissue sample at 8,000 xg for 10 minutes

The evaluation of their cytotoxic activity against tumor cells such as A549, HL-60, MCF-7, colon cancer cells, K562, and HeLa, followed by HepG2, Hep3B, MDA-MB-231, P-388, HT-29, MCF-7, Sup-T1, U937 and other cells, has become the hotspots of research

Because rinsing the nasal cavities with saline is helpful in removing secretions and washing away allergens and irritants, nasal irrigation prior to administration of topical medications can improve the response to the medications (Simon et al., 2015