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Specifically, Fer-1 treatment blocked the increase in ROS, Fe, MDA, and lipid peroxidation, and reversed the decrease in GSH and MMP levels (Fig

Co-immunoprecipitation (co-IP) After treatment, the cells were lysed in an ice-cold co-IP buffer containing 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM EDTA, and 0.5% NP-40, supplemented with a protease inhibitor cocktail (Roche, Cat

Moreover, studies included in this work are often small (Online Supplementary Table S1 (SM)) and thus underpowered, except some exceptions (Hannon et al., Reference Hannon, Dempster, Mansell, Burrage, Bass, Bohlken and Mill2021), which presents the need to create collaborative efforts allowing meta-analysis of comparable epigenetic data

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