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Amplification was performed using a 500/7500 Fast RealTime PCR System (Applied Biosystems) under standard PCR conditions (50 C, 2 min), (95 C 10 min), 40x (95 C, 15 min), (60 C, 1 min)) followed by melting curve acquisition

Ive consumed them for more than 30 years and for a few months Ive noticed that they turn off after they are half gone and cannot be re lighted

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