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In further embodiments, the invention encompasses a method of inhibiting NNMT in vitro or in vivo by contacting a cell expressing NNMT with one or more cations chosen from 1c, 1f, 1l, 1m, 2j, 2k, 2l, 2m, 2aa, 1c, 1f, 1l, 1m, 2j, 2k, 2l, 2m, and 2aa

50mg produces approximately 4050% enzyme inhibition, while 100mg achieves 6075% inhibition, but the curve plateaus beyond 150mg, suggesting diminishing returns

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It enhances lipolysis, which is the breakdown of stored fat in adipose tissue into free fatty acids, making them available for energy use