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Cells were centrifuged and resuspended in Dulbecco modified Eagle medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA, 11995-065) supplemented with 10% heated inactivated fetal bovine serum (FBS, Thermo Fisher Scientific Waltham, MA, USA, 16000-044), 100-U/ml penicillin0.1-mg/ml streptomycin, and 0.25-ng/ml macrophage colony-stimulating factor (Thermo Fisher Scientific Waltham, MA, USA, PHC9504)