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The plates were then washed twice with ice-cold PBS, scraped, pelleted 250 g at 4 C for 3 min and lysed with RIPA buffer (50 mM Tris-Cl (pH = 8), 150 mM NaCl, 5 mM EDTA, 1 % NP-40, 0.5 % sodium deoxycholate, 0.1% SDS, 1x protease and 1x phosphatase inhibitors)

However, since the vital role of this phenomenon in other neurological dysfunction such as Parkinsons disease as mentioned above, it is essential to explore the concrete role of ubiquitination by HDACs for epileptic progression

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Lysyl oxidase, the copper-dependent enzyme that catalyzes the oxidative deamination of lysine residues in collagen and elastin (a step required for the cross-linking that gives mature collagen and elastin their mechanical properties), requires copper as a cofactor