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High-performance liquid chromatography (HPLC) analysis of single erythroid colonies indicated that vector-derived AT87Q -globin expression output did not differ significantly between the HMGA2 integrant and colonies displaying integration at other chromosomal sites [170]

Primary T cells were cultured at 37 C and 5% CO 2 in RPMI supplemented with 10% FBS, 10 mM HEPES, penicillin/streptomycin and 0.004% beta-mercaptoethanol

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Unlike thalidomide, which was once promoted for its extreme safety prior to the discovery of its teratogenicity, acetaminophen has a long history of serious side effects associated with its use (Table 1)