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Concentration (mg/mL) = peptide mass (mg) / volume added (mL)

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To quantify the ciliary basal signal of phospho-(T94)Tctex-1, the total fluorescence of the region of interest, that is, the proximal end of Ac-Tublabeled cilium, was quantified in the projection confocal images of 1.25-m-thick Z-series sections of cilium-containing regions ( x = 1 m, y = 1 m) in randomly selected cells using NIH ImageJ 64 software