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Several candidate non-glutathione disulfide substrates for the dithiol mechanism have been identified using monothiol glutaredoxin mutants as bait proteins for the trapping of Grx(SSR) species (Fig

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Cells were incubated for 10 min at room temperature and then 20 L of 10% CHAPS detergent (Sigma-Aldrich, C3023) was added, and cells incubated at room temperature for an additional 10 min to lyse cells

H2O2Accumulation, host cell death and differential levels of proteins related to photosynthesis, redox homeostasis, and required for viral replication explain the resistance of EMS-mutagenized cowpea to cowpea severe mosaic virus