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Therefore, the addition of balanced amino acids to low-protein diets can improve birds thermotolerance by lowering the energy cost for N excretion (243)

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The ICS was performed as previously described ( Supplementary Table 1 ) and then fixed and permeabilized using a Cytofix/Cytoperm kit (BD Bioscience) or a Transcription Factor Buffer Set (BD Biosciences) and stained with the corresponding antibodies (IFN-, IL-21, IL-10, FoxP3, TCF1, and Eomes)