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For GSSG quantification, the GR-based assay was performed on the neutralized extract following incubation with 2-vinylpyridine as described by The floret concentrations of non-phosphorylated and phosphorylated pyridine dinucleotides were determined with a recycling assay that detected the phenazine methosulfate-based reduction of dichlorophenolindophenol as described by + ) and nicotinamide adenine dinucleotide phosphate (NADP + ) determinations, 100 mg of frozen floret tissue was combined with 1 mL of 0.2 N HCl, vortexed for 5 min and centrifuged at 13,000 g and 4C for 10 min

We calculated the ratios using raw values of these metabolites, ensuring the numerator and denominator were derived from the same three distinct categories as in MGBB-KAS

10.1002/adfm.201907066 56 RabikC

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