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USA 102 , 1399814003 (2005)

Acknowledgments All figures in this article were drawn by Figdraw ( Conflict of interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest

Samples were separated using a Raptor HILIC-Si 2.1 100 mm, 2.7 m LC column (Restek) with a constant flow rate of 400 l/min following a flow gradient starting at 5% Mobile Phase B (H 2 O + 5 mM AmFm +0.5% formic acid) then going up to 40% in 5 min, which was followed by re-equilibration at 5% B for 4 min and 30s

CJC-1295 (29 amino acids) and Ipamorelin (5 amino acids) are produced via solid-phase peptide synthesis, which is far simpler and less expensive