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For controlled skin studies, topical application removes the confounders introduced by injection no injection site reactions, no systemic distribution diluting local concentration, no injection technique variability across research subjects

Beyond Wnt signaling, GHK-Cu has been shown to increase VEGF (vascular endothelial growth factor) expression, which improves blood supply to follicles, and to decrease TGF-beta1, the signaling molecule that triggers follicle regression from anagen into catagen

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