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Average library size and concentration were determined using TapeStation and qPCR (NEBNext Library Quant kit, New England Biolabs), respectively, before pooling

doi: 10.1016/j.chom.2012.06.006 102 SaeedSQuintinJKerstensHHRaoNAAghajanirefahAMatareseFet al

You may need to drink more fluids if you're: pregnant or breastfeeding in a hot environment physically active for long periods ill or recovering from illness Fruit juice and smoothies also count towards your fluid consumption, but they contain free sugars that can damage teeth, so limit these drinks to a combined total of 150ml a day

Biochemical and pharmacological control of the multiplicity of coupling at G-protein-coupled receptors