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Next, 10 L of Mitochondria Isolation Reagent B was added, followed by vortexing at maximum speed for 5 s and incubation on ice for 5 min, with vortexing every minute

Graham et al., 2000)

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While the data showed the presence of oxidative stress and impaired intestinal permeability in the stomach and jejunum tissues in the Rotenone group, these symptoms were observed to be alleviated in the Rotenone + Alpha Pinene group