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Lentivirus production Lentivirus was produced in HEK-293T cells which were transfected using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA) with the plasmid of interest, a packaging plasmid psPAX2, a gift from Didier Trono (Addgene plasmid # 12260

Furthermore, metformin significantly induced necrosis in HCT116 cells, when compared to vehicle control ( Figure 3C )

20,21 Defects in folate metabolism can cause physiological abnormalities associated with ASD such as abnormalities in the purine, methylation and redox metabolic pathways (Fig