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Glycogen synthase kinase 3 (GSK3) in tumorigenesis and cancer chemotherapy

2285-C CHOLESTEROL-ASSOCIATED LOCUS EHBP1 PROTECTS AGAINST NASH FIBROSIS Fanglin Ma and Bishuang Cai, Icahn School of Medicine at Mount Sinai Background: Elevated hepatic cholesterol content is frequently seen in human NASH and high-cholesterol diets have been found to promote NASH progression in mouse models

Aiming for a total peptide amount of 85 g in each sample prior to phosphopeptide enrichment, we directly matched 28 samples to corresponding samples of the full proteome, while 17 samples were combined within groups resulting in a total of 36 samples for phosphoproteomic data acquisition

However, some results differed between these studies, which could be explained by differences in tissue processing and immunostaining