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Moreover, the levels of GPX4 and FTH1 protein measured by western blotting were upregulated in the xenograft mouse model residual tumors after 5-FU treatment ( Figures 5AC , and Supplementary Figure S4A )

To investigate the function of CBS in this process, we silenced its expression by CRISPR/cas9 mediated gene knockout strategy (sg CBS ) in SCD erythroblasts (Fig