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Mitochondrial growth and division during the cell cycle in HeLa cells

Hyland NP, O'Mahony SM, O'Malley D, O'Mahony CM, Dinan TG, Cryan JF (2015) Early-life stress selectively affects gastrointestinal but not behavioral responses in a genetic model of brain-gut axis dysfunction

In the first step, glutamate and cysteine are ligated by -glutamylcysteine synthetase (also known as glutamatecysteine ligase, GCL) to form -glutamylcysteine, utilizing one molecule of ATP

On day 6 of differentiation, the medium was changed to glucose/phenol red/glutamine/pyruvate-free DMEM (Thermo Fisher Scientific) supplemented with 0.5% FCS, 4.5 g l 1 glucose, 4 mM glutamine, 0.5 mM pyruvate and 5 g ml 1 insulin