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Studies indicate that lithium inhibits the expression and activity of the sodium/myo-inositol transporter (SMIT) system, thus limiting the entry of inositol into the cell

After washing with PBS containing 0.3% Tween 20 (Fisher Scientific, Waltham, MA, United States), the NS3-NS5 interaction was detected by incubating the plates with the anti-NS3 monoclonal antibody (GT2811 clone, Genetex, Irvine, CA, United States) diluted 1:6,000 in PBS + 2% (v/v) FBS (Thermo Fisher Scientific) for 1 h at RT

Thus, disruptions caused by Mg deficiency or imbalance in these pathways can adversely affect plant growth, development, and yield (Zhang et al., 2023)

NAD + metabolism: pathophysiologic mechanisms and therapeutic potential