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From pregnancy I to pregnancy III, an increase in the solidified bacterial phylum was observed, which is characteristic of the vaginal microbiome of normal women ( Fusarium , Actinobacteria , Pseudomonas , and Bacillus -like phyla ( Pseudomonas , Bacteroidetes , and Actinobacteria phyla ( It is well-known that there is an interaction between the immune system and the microbiome, involving hormone receptors, various immune mediators, stem cells, different subpopulations of leukocytes, plasma cells, and antibodies such as IgG, IgM, and IgA, along with interleukins and inflammatory proteins

R.ChopadeB

Lane 1: Molecular weight marker Lane 2: Cell pellet after sonication Lane 3: Supernatant after sonication Lane 4: Flow through after binding Lane 5: Wash Lane 6: Elution 1 Lane 7: Elution 2 Lane 8: Elution 3 Lane 9: Elution 4 Lane 10: Elution 5 GST-E2-25K was purified with Glutathione Agarose Resin using a gravity column

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