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For IC 50 calculations with dengue virus, phenformin or atpenin A5 were prediluted in Huh7.5 complete media and added to Huh7.5 cells 1 h before adding the virus (MOI = 1)

Conversely, in another research applying stem cell educator therapy [49], among the nine subjects, only one individual with AU failed to experience hair regrowth, who had previously accepted long-term treatment of oral prednisone