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On the day of the experiment, a fresh inhibitor plate was thawed and diluted 25-fold into DMEM in a 384 deep well daughter plate (Greiner), using a Biomek FX

In addition, the nanovaccine Si9GM is made of central radial mesoporous silica nanoparticles coated with membranes from bone marrow-derived dendritic cells (BMDCs) expressing antigen peptides, used to deliver CLEC9A-antigen conjugates and STING agonists (23-cGAMP), which can selectively target cDC1, promote DCs maturation, and lead to upregulation of cytotoxic T cells, reduction of Tregs, and M1/M2 macrophage polarization and can induce strong tumor growth and metastasis inhibition when combined with PD-1 blockade (224)

Ann Neurol 53(S3):S26S38 Katayama M, Mukai Y, Taniguchi H (1987) Determination of aromatic aldehydes by high performance liquid chromatography after precolumn fluorescent derivatization with 4, 5-dimethyl-o-phenylenediamine