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Subsequently, 0.1 mM IPTG was added to the transfected cells, which was then cultured with 1 L LB medium for 16 h at 27 C

*Correspondence: Anna Bendarska-Czerwiska, [email protected]

A post-absorptive state ensures digestive demands are not competing with the peptides cellular signaling, allowing for cleaner mitochondrial activation

The column was eluted isocratically at a flow rate of 450 L/min for 1 minute at 80% mobile-phase A (10 mM NH 4 Ac/methanol/acetic acid 95:5:0.1, by volume), followed by a linear gradient to 80% mobile-phase B (methanol/acetic acid