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The resulting supernatant was collected, and protein concentrations were determined using a BCA assay kit (Thermo Fisher Scientific)

Cirrhosis mortality rates vary substantially among age groups: They are very low among young people but increase considerably in middle age

ii) Primary culture of satellite cells with 5 ng/ml HGF, which received only the LASSS pre-treatment (at 1:8000, without peroxynitrite treatment), exhibited a cell-proliferation activity (evaluated by mRNA expression levels of MyoD and its counter-regulatory factor myogenin at 48-h and 72-h time-points) comparable to that of the positive control culture treated with 5 ng/ml control HGF without LASSS (just with the solvent