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PARP-1 inhibition increases mitochondrial metabolism through SIRT1 activation

Liver diseases have been widely studied in the context of these enzymes that either oxidatively metabolize ethanol into toxic metabolites, or detoxify alcohol and are made less effective through genetic polymorphisms

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Cells were incubated with the following secondary antibodies in 1% BSA for 1 hour at room temperature in the dark: Alexa Fluor 488 (1:1000, ab150073, Abcam or R10477, Thermofisher Scientific) and goat anti-mouse conjugated secondary antibody (Texas Red, 1:1000, ab6787, Abcam)