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Sci Rep 10:14989

RNA quality and quantity was determined using 1% agarose gel electrophoresis, NanoDrop Photometer Spectrophotometer (IMPLEN, Westlake Village, CA, USA), Qubit RNA Assay Kit in Qubit 2.0 Flurometer (Life Technologies, Carlsblad, CA, USA), and RNA Nano 6000 Assay Kit of the Bioanalyser 2100 system (Agilent Technologies, Santa Clara, CA, USA)

Furthermore, serum T3 levels rose in both treated groups, while serum T4 levels dropped in the three groups after the trial

Additionally, no detectable toxicity was reported at therapeutic doses and the compound exhibited excellent oral bioavailability and blood-brain barrier (BBB) penetrance