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Lyophilized powder stores at 20C and remains stable for 24+ months when kept sealed and away from light

Except there's one other important consideration for making HSA contributions after 65, which is that along with not signing up for Medicare, an individual needs to also avoid applying for Social Security benefits, since doing so will automatically enroll them in Medicare and, therefore, end their eligibility to make HSA contributions

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Preparation of cell extracts and western blot analysis Cells were trypsinized, washed with PBS and resuspended in lysis buffer [50 mM TrisHCl (pH 7.5), 120 mM NaCl, 0.5% (v/v) Nonidet P-40] supplemented with protease inhibitor cocktail (Roche, 11873580001) and incubated for 5 min on ice