glutathione peroxidase assay kit biovision (Colorimetric) ensuring smooth and accurate processing Sigma-Aldrich Glutathione Peroxidase Assay Kit,

doi: 10.1002/adma.202108174 168 ZhangLZhangBZhangM-JLiWLiHJiaoYet al

AMS binds to reduced thiol groups in Cys residues and increases the molecular mass of the protein, so that the reduced and oxidized forms of GSAAT are readily distinguishable on SDS-PA gels, since the labeled protein (reduced form) migrates more slowly than the unlabeled oxidized form

For comparisons between two groups, Student's t-test was used if the data were normally distributed and had homogeneity of variance

If the original packaging is not available, wrap the vial in aluminum foil to block light and place it in a small zip-lock bag to protect against moisture from refrigerator humidity