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Maintenance energy requirements were calculated using an average energetic intake estimated for each cat based on individual feed intakes required to maintain an ideal BCS

O 2 consumption Consumption of O 2 was measured using an ISO-OXY-2 O 2 sensor attached to an APOLLO-4000 free radical analyzer (World Precision Instruments, Inc., Sarasota, FL) as described [22]

Interestingly, genetic mutations in sALS patients have also been described, in C9orf72 (7% of cases), TARDBP, SOD1, FUS, VCP, p62, PFN-1 , MATR3, OPTN, UBQLN2 , CHCHD10, TBK1 , TUBA4A, NEK1, C21orf2 , and CCNF , together accounting for 15% of sporadic ALS cases (Taylor et al., 2016