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In some embodiments, a buffer solution is mixed at a flow rate ranging from about 100-6000 ml/minute (e.g., about 100-300 ml/minute, 300-600 ml/minute, 600-1200 ml/minute, 1200-2400 ml/minute, 2400-3600 ml/minute, 3600-4800 ml/minute, 4800-6000 ml/minute, or 60-420 ml/minute)

Plasma concentrations of insulin and leptin were measured using a custom developed Mesoscale (MSD) multiplex assay, which is a multi-array assay with electrochemiluminescence readout

A 2022 study in Frontiers in Pharmacology reported absorption, distribution, metabolism, and excretion data for the compound in rats and dogs, providing one of the first structured pharmacokinetic profiles in the peer-reviewed record [7]