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To investigate this question, we varied the amino acid input (cysteine, glycine, glutamate, methionine, and serine) throughout the day and used the model to compute the time courses of all the concentrations in the model, and all the velocities

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After 9 h incubation, the supernatants were collected by centrifugation and the release of ATP was detected by the luminescent ATP detection assay kit as described in the manufactures protocols