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10.1016/j.ecoenv.2023.115035 186 ZhangX.XuW.ZhongW.ZhangW.YangC.DuanL.et al

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Bacterial cells were harvested, washed three times with sterile saline, then collected by centrifugation at 8,000 rpm at 4C for 5 min, and immediately quenched with liquid nitrogen