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Undermethylation contributes to predictable traits: high internal tension, strong preference for structure, difficulty shifting attention, and repetitive or ritualistic behaviors that serve as self-soothing

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After 4-day culture, organoids were dissociated in organoid dissociation solution (bioGenous, E238001) for 2 min at 37 C according to the manufacturers protocol, centrifuged at 68 g for 2 min, resuspended in organoid culture medium, and cultured as described above for organoids passaging