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Conversely, a V203A point mutation disrupting TRAF1/cIAP2 interaction blocks TAK1 phosphorylation downstream of TLR4, inhibiting NF-B/MAPK pathway activation and reducing joint inflammation in rheumatoid arthritis models (292)

Supernatants were diluted with ChIP dilution buffer (50 mM Tris-HCl [pH 8.0], 167 mM NaCl, 1.1% Triton X-100, 0.11% NaDoc, 200 nM PMSF), precleared with normal rabbit IgG (Cell Signaling Technology, #2729) or normal mouse IgG (Santa Cruz, sc-2025), and incubated with Protein G agarose beads (Thermo Scientific, 20398) pre-washed in 3.125 mg/mL BSA/ChIP dilution buffer for 2 h at 4 C

Effect of Aged Garlic Ethyl Acetate Extract on Oxidative Stress and Cholinergic Function of Scopolamine-Induced Cognitive Impairment in Mice

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