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The lack of conserved markers hampers clinical assessment and underscores the need for human-centric validation pipelines that integrate single-cell transcriptomic and imaging datasets with body fluid biomarkers

By using the ability of puromycin to incorporate into protein during synthesis and our ability to detect it with an anti-puromycin antibody, the SCENITH method utilizes the level of puromycin incorporation as a marker of protein synthesis and thus, a surrogate marker of cell global metabolic activity (Figure 2B)

Effects of Dietary Phytoestrogens on Hormones throughout a Human Lifespan: A Review

Mouse primary bone marrow (BM)-derived macrophages (BMDMs) were prepared and cultured following the previous protocols 63