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Protein preparation and Western blot analysis Cultured cells or flashfrozen tissues were homogenized in icecold radioimmunoprecipitation assay (RIPA) buffer (50 mM TrisHCl pH 7.6, 150 mM NaCl, 0.5% sodium deoxycholate, 1% NP40, 0.1% SDS, 2 mM EDTA) containing COMPLETE protease inhibitor cocktail (Roche) and phosphatase inhibitors (Santa Cruz Biotechnology)

Accelerated degradation is probably secondary to hypermetabolism and is not a primary event (269), although it has been reported that accelerated T4 turnover can persist after treatment of thyrotoxicosis (270)

2022-1038 is hereby updated to remove the aforementioned food supplement