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Mueller-Hinton Broth (Oxoid, United Kingdom) or Mueller-Hinton Broth with 50% plasma was used to prepare a series of two-fold dilutions of the appropriate antimicrobial substance, ranging from 0.25 g/mL to 64 g/mL

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This solution was subsequently modified [2] to provide a simple method for the preparation of mononuclear cells (MCs) from rodent and rabbit blood, which have a higher density than those from human blood