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Elution of specifically bound phage particles from glutathione Sepharose was carried out with glutathione buffer (20 mM glutathione, 100 mM Tris, 200 mM NaCl, 0.1% Tween 20, pH 8.0) or for Ni-NTA agarose was carried out competitively with buffer with 500 mM imidazole (500 mM imidazole, 50 mM Na 2 HPO 4 , 300 mM NaCl, 0.1% Tween 20, pH 7.5)

The efficacy evidence for edaravone is primarily derived from clinical trials in East Asian populations, and its benefits have been less consistently replicated in Western studies and real-world settings outside of Japan

(2012) Guidelines for the use and interpretation of assays for monitoring autophagy

World J Gastroenterol 2014;20:11271138.ArticlePubMedPMC 23