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Lowering the pH causes a shift toward the tetrameric form

The conditions, such as a 75 mM sodium phosphate buffer (pH 3.0) with 1.7 mM 1-octanesulfonic acid, 25 M EDTA, 10% methanol, and a flow rate of 1 mL/min, are optimized for precision and sensitivity, ensuring accurate neurotransmitter profiling at a column temperature of 25C

Another study successfully expressed and secreted PR-FO, a different AMPs, achieving yields of 16 mg/L and 23 mg/L of fusion protein using SP amyQ and SP sacB signal peptides, respectively (Zhang et al., 2020)

doi: 10.1128/spectrum.01730-22 60 FiorentinoGCoppolaAIzzoRAnnunziataABernardoMLombardiAet al