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After incubation in the dark at 4 C for 2 days, plates were cultivated in a growth chamber at 22 C with a 16 h/8 h light/dark (long day, LD) photoperiod

Depletion of both CBS and CSE causes hyperhomocystenaemia, which is an independent risk factor for developing cardiovascular deficits and neurodegenerative disorders such as Alzheimer's disease (Mattson and Shea, 2003