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The insoluble pellet, which contained nuclei, was resuspended in 50 l Complete Lysis Buffer and vortexed for 15 s, incubated and suspended for 30 min on ice on a rocking platform set at 150 rpm, then vortexed again for 30 s and centrifuged at 14,000 g for 20 min at 4 C

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Photoelectric smoke detectors are generally more likely to react to vaping because they detect airborne particles using a light sensor