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S.TamhaneV

It is important to note that these metabolic responses to SAAR are not confined to rodents

In particular, external (cytoplasmic) Cl concentrations exhibit a biphasic effect on glutamate transport with maximal activity in the presence of 430 mM Cl , depending on glutamate concentrations used for the transport assay (40 M and 5 mM glutamate, respectively

1- Free GSH=DTNB+Sample+Substrate 2- Total GSH=DTNB+Sample+Substrate+ NADPH+glutathione reductase 3- GSSG=Total GSH-Free GSH Another method to measure GSSG separately, is to remove the free GSH from DTNB reaction process by modification of free GSH with N-ethylmaleimide (NEM) or vinylpyridine prior to conducting assay